EXOSC10 ELISA Kit (Human)
Product Specifications
Reproducibility
Mean Intra-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //
CAS Number
7732-18-5
Gene Name
Exosome component 10
Gene Aliases
Autoantigen PM/Scl 2; autoantigen PM-SCL; exosome component 10; P100 polymyositis-scleroderma overlap syndrome-associated autoantigen; p2; p3; p4; PM/Scl-100; PM-Scl; PMSCL; PMSCL2; polymyositis/scleroderma autoantigen 100 kDa; polymyositis/scleroderma autoantigen 2; RRP6; Rrp6p.
Gene ID
5394
Accession Number
NP_001001998.1
Reactivity
Homo sapiens, Human
Target
Exosome component 10
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology EXOSC10 ELISA Kit (Human) (OKEH08569) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for EXOSC10 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for EXOSC10 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample EXOSC10 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Detection Range
31.2-2000pg/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 110% (n = 20)
Sensitivity
10.2pg/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Human Exosome component 10
NCBI Gene Symbol
EXOSC10
Protein Name
Q01780
Gene Name URL
EXOSC10
Nucleotide Accession Number
NM_001001998.2
Frequently Asked Questions
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