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EXOSC9 ELISA Kit (Human)

Product Specifications

Reproducibility

Mean Intra-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //

CAS Number

7732-18-5

Gene Name

Exosome component 9

Gene Aliases

Autoantigen PM/Scl 1; exosome complex component RRP45; exosome complex exonuclease RRP45; Exosome component 9; p5; p6; P75 polymyositis-scleroderma overlap syndrome associated autoantigen; P75 polymyositis-scleroderma overlap syndrome-associated autoantigen; PCH1D; PM/Scl-75; PMSCL autoantigen, 75kD; PMSCL1; Polymyositis/scleroderma autoantigen 1; polymyositis/scleroderma autoantigen 1, 75kDa; Polymyositis/scleroderma autoantigen 75 kDa; RRP45; Rrp45p.

Gene ID

5393

Accession Number

NP_001029366.1

Reactivity

Homo sapiens, Human

Target

Exosome complex component RRP45

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology EXOSC9 ELISA Kit (Human) (OKEH08568) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for EXOSC9 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for EXOSC9 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample EXOSC9 captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Detection Range

0.156-10ng/mL

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 83% (n = 20)

Sensitivity

0.055ng/mL

Reconstitution

Store as indicated in product manual.

Specificity

Natural and recombinant Human Exosome complex component RRP45

NCBI Gene Symbol

EXOSC9

Protein Name

Q06265

Gene Name URL

EXOSC9

Nucleotide Accession Number

NM_001034194.1

Frequently Asked Questions

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