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RPS6KB1 ELISA Kit (Rat)

Selected Variant

OKEH08539-96W

Size: 96 Tests

Product Specifications

Reproducibility

Mean Intra-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //

CAS Number

7732-18-5

Gene Name

Ribosomal protein S6 kinase B1

Gene Aliases

70 kDa ribosomal protein S6 kinase 1; p70 ribosomal S6 kinase alpha; p70 S6 kinase alpha; p70 S6KA; p70 S6K-alpha; p70 (S6K) -alpha; P70S6K; p70-S6K 1; P70S6K1; ribosomal protein S6 kinase beta-1; ribosomal protein S6 kinase I; ribosomal protein S6 kinase, 70kDa, polypeptide 1; ribosomal protein S6 kinase, polypeptide 1; S6 kinase; S6K; S6K1; S6K-beta-1.

Gene ID

83840

Accession Number

NP_114191.1

Reactivity

Rat, Rattus norvegicus

Target

Ribosomal protein S6 kinase beta-1

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology RPS6KB1 ELISA Kit (Rat) (OKEH08539) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for RPS6KB1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for RPS6KB1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample RPS6KB1 captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Detection Range

0.156-10ng/mL

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 101% (n = 20)

Sensitivity

0.078ng/mL

Reconstitution

Store as indicated in product manual.

Specificity

Natural and recombinant Rat Ribosomal protein S6 kinase beta-1

NCBI Gene Symbol

Rps6kb1

Protein Name

P67999

Gene Name URL

Rps6kb1

Nucleotide Accession Number

NM_031985.1

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