PRKN ELISA Kit (Rat)
Product Specifications
Reproducibility
Mean Intra-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //
CAS Number
7732-18-5
Gene Name
Parkin RBR E3 ubiquitin protein ligase
Gene Aliases
E3 ubiquitin-protein ligase parkin; Park; Park2; parkin RBR E3 ubiquitin protein ligase 2; Parkin RBR E3 ubiquitin-protein ligase; parkin variant SV5DEL; Parkinson disease (autosomal recessive, juvenile) 2, parkin; parkinson protein 2, E3 ubiquitin protein ligase.
Gene ID
56816
Accession Number
NP_064478.1
Reactivity
Rat, Rattus norvegicus
Target
E3 ubiquitin-protein ligase parkin
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology PRKN ELISA Kit (Rat) (OKEH08514) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for PRKN has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for PRKN is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample PRKN captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Detection Range
15.6-1000pg/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 97% (n = 20)
Sensitivity
10pg/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Rat E3 ubiquitin-protein ligase parkin
NCBI Gene Symbol
Prkn
Protein Name
Q9JK66
Gene Name URL
Prkn
Nucleotide Accession Number
NM_020093.1
Frequently Asked Questions
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