What is the catalog number for UCP1 ELISA Kit (Dog)?

The catalog number for UCP1 ELISA Kit (Dog) is OKEH08476-96W.

What sizes are available for UCP1 ELISA Kit (Dog)?

UCP1 ELISA Kit (Dog) is available in the following size(s): 96 Tests.

Is UCP1 ELISA Kit (Dog) in stock?

UCP1 ELISA Kit (Dog) is currently made to order / not held in immediate stock. Contact us for lead time.

What should I do if I need to report a performance issue with a specific lot of this product?

Provide the catalogue number, product name, lot number, and your experimental results to our technical support team. They’ll investigate and, if needed, escalate to the manufacturer, provided the product was used before expiry and stored correctly.

What is the isotype of this antibody?

Isotype information such as IgG1 or IgG2a is listed in the product specifications on the datasheet, relevant for selecting compatible detection reagents.

Can I get a trial-size or discounted first order of this product?

We don’t generally offer free samples, but trial discounts in exchange for feedback and validation results are almost always possible. Contact us with your intended application to arrange one.

UCP1 ELISA Kit (Dog)

Product Specifications

Reproducibility

Mean Intra-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //

CAS Number

7732-18-5

Gene Name

Uncoupling protein 1

Gene Aliases

Mitochondrial brown fat uncoupling protein 1; solute carrier family 25 member 7; thermogenin; UCP 1; uncoupling protein 1 UCP1.

Gene ID

403574

Accession Number

NP_001003046.1

Reactivity

Canis lupus, Dog

Target

Mitochondrial brown fat uncoupling protein 1

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology UCP1 ELISA Kit (Dog) (OKEH08476) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for UCP1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for UCP1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample UCP1 captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Detection Range

78-5000pg/mL

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 119% (n = 20)

Sensitivity

34pg/mL

Reconstitution

Store as indicated in product manual.

Specificity

Natural and recombinant Dog Mitochondrial brown fat uncoupling protein 1

NCBI Gene Symbol

UCP1

Protein Name

Q9GMZ1

Gene Name URL

UCP1

Nucleotide Accession Number

NM_001003046.1

Frequently Asked Questions

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