What is the catalog number for GNRHR ELISA Kit (Pig)?

The catalog number for GNRHR ELISA Kit (Pig) is OKEH08409-96W.

What sizes are available for GNRHR ELISA Kit (Pig)?

GNRHR ELISA Kit (Pig) is available in the following size(s): 96 Tests.

Is GNRHR ELISA Kit (Pig) in stock?

GNRHR ELISA Kit (Pig) is currently made to order / not held in immediate stock. Contact us for lead time.

Is this kit compatible with automated liquid handling platforms?

Automation compatibility varies by kit and isn’t always listed on the datasheet. Contact our technical support team with details of your platform so we can confirm with the manufacturer.

What applications has this product been validated for?

Validated applications such as WB, ELISA, IHC, ICC, or FC are listed on the product datasheet. If you need application-specific validation data before ordering, contact our technical support team with the catalogue number.

How do I reconstitute this lyophilized product?

Reconstitution instructions, including the recommended diluent and final concentration, are provided on the datasheet. Aliquot immediately after reconstitution to avoid repeated freeze-thaw cycles.

GNRHR ELISA Kit (Pig)

Product Specifications

Reproducibility

Mean Intra-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //

CAS Number

7732-18-5

Gene Name

Gonadotropin releasing hormone receptor

Gene Aliases

GnRH receptor; gnRH-R; gonadotropin-releasing hormone receptor; LHRH; luteinizing hormone-releasing hormone receptor.

Gene ID

397515

Accession Number

NP_999438.1

Reactivity

Porcine, Sus scrofa

Target

Gonadotropin-releasing hormone receptor

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Competitive

Assay Principle

Aviva Systems Biology GNRHR ELISA Kit (OKEH08409) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-GNRHR antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated GNRHR and incubated. The GNRHR found in the sample or standards competes with the biotinylated GNRHR for limited binding sites on the immobilized anti-GNRHR antibody. Excess unbound biotinylated GNRHR and sample or standard GNRHR is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated GNRHR captured in the well and inversely proportional to the amount of GNRHR which was contained in the sample or standard.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol