What is the catalog number for TFF3 ELISA Kit (Dog)?

The catalog number for TFF3 ELISA Kit (Dog) is OKEH08404-96W.

What sizes are available for TFF3 ELISA Kit (Dog)?

TFF3 ELISA Kit (Dog) is available in the following size(s): 96 Tests.

Is TFF3 ELISA Kit (Dog) in stock?

TFF3 ELISA Kit (Dog) is currently made to order / not held in immediate stock. Contact us for lead time.

What buffer is this product supplied in?

The formulation or storage buffer is listed on the product datasheet. If you require a carrier-free or custom formulation, contact us before ordering, as this may involve a made-to-order production timeline.

What dilution range is recommended for immunohistochemistry (IHC) with this antibody?

A starting IHC dilution range is listed on the datasheet, but should be optimized for your specific tissue type and staining protocol.

Can I request a Safety Data Sheet (SDS) for this product?

Yes, safety data sheets are available for hazardous or chemically classified products and can be provided on request or downloaded from the product page.

TFF3 ELISA Kit (Dog)

Selected Variant

OKEH08404-96W

Size: 96 Tests

Product Specifications

Reproducibility

Mean Intra-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //

CAS Number

7732-18-5

Gene Name

Trefoil factor 3

Gene Aliases

Intestinal trefoil factor; ITF; trefoil factor 3; trefoil factor family peptide 3.

Gene ID

403488

Accession Number

NP_001002990.1

Reactivity

Canis lupus, Dog

Target

Trefoil factor 3

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology TFF3 ELISA Kit (Dog) (OKEH08404) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for TFF3 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for TFF3 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample TFF3 captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Detection Range

0.78-50ng/mL

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 109% (n = 20)

Sensitivity

0.394ng/mL

Reconstitution

Store as indicated in product manual.

Specificity

Natural and recombinant Dog Trefoil factor 3

NCBI Gene Symbol

TFF3

Protein Name

Q863B4

Gene Name URL

TFF3

Nucleotide Accession Number

NM_001002990.1

Price

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