ID1 ELISA Kit (Rat)
Product Specifications
Reproducibility
Mean Intra-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //
CAS Number
7732-18-5
Gene Name
Inhibitor of DNA binding 1, HLH protein
Gene Aliases
DNA-binding protein inhibitor ID-1; ID125A; Idb1; inhibitor of differentiation 1; Inhibitor of DNA binding 1; Inhibitor of DNA binding 1 helix-loop-helix protein (splice varaiation) ; inhibitor of DNA binding 1, dominant negative helix-loop-helix protein; Inhibitor of DNA binding 1, helix-loop-helix protein (splice variation) .
Gene ID
25261
Accession Number
NP_036929.2
Reactivity
Rat, Rattus norvegicus
Target
DNA-binding protein inhibitor ID-1
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology ID1 ELISA Kit (Rat) (OKEH08359) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for ID1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for ID1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample ID1 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Detection Range
0.312-20 ng/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 97% (n = 20)
Sensitivity
0.082ng/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Rat DNA-binding protein inhibitor ID-1
NCBI Gene Symbol
Id1
Protein Name
P41135
Gene Name URL
Id1
Nucleotide Accession Number
NM_012797.2
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