GDF7 ELISA Kit (Human)
Product Specifications
Reproducibility
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CAS Number
7732-18-5
Gene Name
Growth differentiation factor 7
Gene Aliases
BMP12; GDF-7; growth/differentiation factor 7.
Gene ID
151449
Accession Number
NP_878248.2
Reactivity
Homo sapiens, Human
Target
Growth/differentiation factor 7
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology GDF7 ELISA Kit (Human) (OKEH08350) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for GDF7 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for GDF7 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample GDF7 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Detection Range
78-5000pg/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 105% (n = 20)
Sensitivity
23pg/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Human Growth/differentiation factor 7
NCBI Gene Symbol
GDF7
Protein Name
Q7Z4P5
Gene Name URL
GDF7
Nucleotide Accession Number
NM_182828.3
Frequently Asked Questions
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