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GNPNAT1 ELISA Kit (Mouse)

Product Specifications

Reproducibility

Mean Intra-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //

CAS Number

7732-18-5

Gene Name

Glucosamine-phosphate N-acetyltransferase 1

Gene Aliases

AU017428; AU040593; EMeg32; glucosamine 6-phosphate N-acetyltransferase; glucosamine-6-phosphate acetyltransferase; glucosamine-phosphate N-acetyltransferase 1; glucosamine-6-phosphate acetyltransferase; glucosamine-phosphate N-acetyltransferase; Gpnat1; Gsnpat; phosphoglucosamine acetylase; phosphoglucosamine transacetylase; Protein EMeg32.

Gene ID

54342

Accession Number

NP_062298.1

Reactivity

Mouse, Mus musculus

Target

Glucosamine 6-phosphate N-acetyltransferase

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology GNPNAT1 ELISA Kit (Mouse) (OKEH08315) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for GNPNAT1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for GNPNAT1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample GNPNAT1 captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Detection Range

0.625-40ng/mL

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 102% (n = 20)

Sensitivity

0.265ng/mL

Reconstitution

Store as indicated in product manual.

Specificity

Natural and recombinant Mouse Glucosamine 6-phosphate N-acetyltransferase

NCBI Gene Symbol

Gnpnat1

Protein Name

Q9JK38

Gene Name URL

Gnpnat1

Nucleotide Accession Number

NM_019425.2

Frequently Asked Questions

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