SCN8A ELISA Kit (Human)

Product Specifications

Reproducibility

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CAS Number

7732-18-5

Gene Name

Sodium voltage-gated channel alpha subunit 8

Gene Aliases

BFIS5; CERIII; CIAT; DEE13; EIEE13; hNa6/Scn8a voltage-gated sodium channel; MED; MYOCL2; NaCh6; Nav1.6; PN4; sodium channel protein type 8 subunit alpha; Sodium channel protein type VIII subunit alpha; sodium channel, voltage gated, type VIII, alpha subunit; voltage-gated sodium channel subunit alpha Nav1.6; voltage-gated sodium channel type VIII alpha protein.

Gene ID

6334

Accession Number

NP_001171455.1

Reactivity

Homo sapiens, Human

Target

Sodium channel protein type 8 subunit alpha

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology SCN8A ELISA Kit (Human) (OKEH08292) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for SCN8A has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for SCN8A is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample SCN8A captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol