What is the catalog number for HMCES ELISA Kit (Mouse)?

The catalog number for HMCES ELISA Kit (Mouse) is OKEH08281-96W.

What sizes are available for HMCES ELISA Kit (Mouse)?

HMCES ELISA Kit (Mouse) is available in the following size(s): 96 Tests.

Is HMCES ELISA Kit (Mouse) in stock?

HMCES ELISA Kit (Mouse) is currently made to order / not held in immediate stock. Contact us for lead time.

HMCES ELISA Kit (Mouse)

Product Specifications

Reproducibility

Mean Intra-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //

CAS Number

7732-18-5

Gene Name

5-hydroxymethylcytosine (hmC) binding, ES cell specific

Gene Aliases

8430410A17Rik; abasic site processing protein HMCES; C85376; embryonic stem cell-specific 5-hydroxymethylcytosine-binding protein; ES cell-specific 5hmC-binding protein; peptidase HMCES; putative endonuclease HMCES; putative peptidase SRAPD1; S; SRAP domain-containing protein 1; Srap1; UPF0361 protein C3orf37 homolog.

Gene ID

232210

Accession Number

NP_776098.1

Reactivity

Mouse, Mus musculus

Target

Embryonic stem cell-specific 5-hydroxymethylcytosine-binding protein

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology HMCES ELISA Kit (Mouse) (OKEH08281) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for HMCES has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for HMCES is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample HMCES captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol