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P3H1 ELISA Kit (Rat)

Product Specifications

Reproducibility

Mean Intra-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //

CAS Number

7732-18-5

Gene Name

Prolyl 3-hydroxylase 1

Gene Aliases

Gros1; growth supressor 1; Lepre1; leprecan-1; leucine- and proline-enriched proteoglycan 1; leucine proline-enriched proteoglycan (leprecan) 1; prolyl 3-hydroxylase 1.

Gene ID

114200

Accession Number

NP_446119.1

Reactivity

Rat, Rattus norvegicus

Target

Prolyl 3-hydroxylase 1

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology P3H1 ELISA Kit (Rat) (OKEH08274) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for P3H1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for P3H1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample P3H1 captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Detection Range

0.312-20ng/mL

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 103% (n = 20)

Sensitivity

0.168ng/mL

Reconstitution

Store as indicated in product manual.

Specificity

Natural and recombinant Rat Prolyl 3-hydroxylase 1

NCBI Gene Symbol

P3h1

Protein Name

Q9R1J8

Gene Name URL

P3h1

Nucleotide Accession Number

NM_053667.1

Frequently Asked Questions

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