What is the catalog number for SRD5A1 ELISA Kit (Bovine)?

The catalog number for SRD5A1 ELISA Kit (Bovine) is OKEH08265-96W.

What sizes are available for SRD5A1 ELISA Kit (Bovine)?

SRD5A1 ELISA Kit (Bovine) is available in the following size(s): 96 Tests.

Is SRD5A1 ELISA Kit (Bovine) in stock?

SRD5A1 ELISA Kit (Bovine) is currently made to order / not held in immediate stock. Contact us for lead time.

Can this product be used for co-immunoprecipitation (Co-IP)?

Suitability for Co-IP depends on the specific antibody and is noted under validated applications on the datasheet. If it isn’t listed, contact our technical support team for guidance based on your target and sample type.

Is this kit compatible with automated liquid handling platforms?

Automation compatibility varies by kit and isn’t always listed on the datasheet. Contact our technical support team with details of your platform so we can confirm with the manufacturer.

What applications has this product been validated for?

Validated applications such as WB, ELISA, IHC, ICC, or FC are listed on the product datasheet. If you need application-specific validation data before ordering, contact our technical support team with the catalogue number.

SRD5A1 ELISA Kit (Bovine)

Product Specifications

Reproducibility

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CAS Number

7732-18-5

Gene Name

Steroid 5 alpha-reductase 1

Gene Aliases

3-oxo-5-alpha-steroid 4-dehydrogenase 1; S5AR 1; SR type 1; Steroid 5-alpha-reductase 1.

Gene ID

614612

Accession Number

NP_001092607.1

Reactivity

Bos taurus, Bovine

Target

3-oxo-5-alpha-steroid 4-dehydrogenase 1

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Competitive

Assay Principle

Aviva Systems Biology SRD5A1 ELISA Kit (OKEH08265) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-SRD5A1 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated SRD5A1 and incubated. The SRD5A1 found in the sample or standards competes with the biotinylated SRD5A1 for limited binding sites on the immobilized anti-SRD5A1 antibody. Excess unbound biotinylated SRD5A1 and sample or standard SRD5A1 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated SRD5A1 captured in the well and inversely proportional to the amount of SRD5A1 which was contained in the sample or standard.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol