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POU2F3 ELISA Kit (Human)

Product Specifications

Reproducibility

Mean Intra-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //

CAS Number

7732-18-5

Gene Name

POU class 2 homeobox 3

Gene Aliases

Epoc-1; OCT11; OCT-11; octamer-binding protein 11; octamer-binding transcription factor 11; OTF-11; PLA1; PLA-1; POU domain transcription factor OCT11a; POU domain, class 2, transcription factor 3; Skn-1a; transcription factor PLA-1; transcription factor Skn-1.

Gene ID

25833

Accession Number

NP_001231611.1

Reactivity

Homo sapiens, Human

Target

POU domain, class 2, transcription factor 3

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology POU2F3 ELISA Kit (Human) (OKEH08260) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for POU2F3 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for POU2F3 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample POU2F3 captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Detection Range

0.312-20ng/mL

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 88% (n = 20)

Sensitivity

0.169ng/mL

Reconstitution

Store as indicated in product manual.

Specificity

Natural and recombinant Human POU domain, class 2, transcription factor 3

NCBI Gene Symbol

POU2F3

Protein Name

Q9UKI9

Gene Name URL

POU2F3

Nucleotide Accession Number

NM_001244682.1

Frequently Asked Questions

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