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RAC1 ELISA Kit (Mouse)

Product Specifications

Reproducibility

Mean Intra-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //

CAS Number

7732-18-5

Gene Name

Rac family small GTPase 1

Gene Aliases

AL023026; D5Ertd559; D5Ertd559e; p21-Rac1; RAS-related C3 botulinum substrate 1; ras-related C3 botulinum toxin substrate 1.

Gene ID

19353

Accession Number

NP_033033.1

Reactivity

Mouse, Mus musculus

Target

Ras-related C3 botulinum toxin substrate 1

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology RAC1 ELISA Kit (Mouse) (OKEH08234) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for RAC1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for RAC1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample RAC1 captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Detection Range

0.156-10ng/mL

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 94% (n = 20)

Sensitivity

0.085ng/mL

Reconstitution

Store as indicated in product manual.

Specificity

Natural and recombinant Mouse Ras-related C3 botulinum toxin substrate 1

NCBI Gene Symbol

Rac1

Protein Name

P63001

Gene Name URL

Rac1

Nucleotide Accession Number

NM_009007.2

Frequently Asked Questions

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