DEGS1 ELISA Kit (Human)
Product Specifications
Reproducibility
Mean Intra-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //
Gene Name
Delta 4-desaturase, sphingolipid 1
Gene Aliases
Cell migration-inducing gene 15 protein; Degenerative spermatocyte homolog 1; degenerative spermatocyte homolog 1, lipid desaturase; degenerative spermatocyte homolog, lipid desaturase; DEGS; DEGS-1; Des-1; DES1; dihydroceramide desaturase 1; FADS7; HLD18; membrane fatty acid (lipid) desaturase; membrane lipid desaturase; MIG15; migration-inducing gene 15 protein; MLD; sphingolipid delta 4 desaturase; sphingolipid delta (4) -desaturase 1; sphingolipid delta (4) -desaturase DES1.
Gene ID
8560
Accession Number
NP_003667.1
Reactivity
Homo sapiens, Human
Target
Sphingolipid delta (4) -desaturase DES1
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology DEGS1 ELISA Kit (Human) (OKEH08228) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for DEGS1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for DEGS1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample DEGS1 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Detection Range
78-5000pg/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 90% (n = 20)
Sensitivity
40.2pg/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Human Sphingolipid delta (4) -desaturase DES1
NCBI Gene Symbol
DEGS1
Protein Name
O15121
Gene Name URL
DEGS1
CAS Number
7732-18-5
Nucleotide Accession Number
NM_003676.3
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