What is the catalog number for FEN1 ELISA Kit (Human)?

The catalog number for FEN1 ELISA Kit (Human) is OKEH08213-96W.

What sizes are available for FEN1 ELISA Kit (Human)?

FEN1 ELISA Kit (Human) is available in the following size(s): 96 Tests.

Is FEN1 ELISA Kit (Human) in stock?

FEN1 ELISA Kit (Human) is currently made to order / not held in immediate stock. Contact us for lead time.

Can I get a trial-size or discounted first order of this product?

We don’t generally offer free samples, but trial discounts in exchange for feedback and validation results are almost always possible. Contact us with your intended application to arrange one.

Do you offer bulk or custom quantities of this product?

Yes. Ordering two or more units, or combining this with other products, usually qualifies for a discount depending on quantity. Contact our sales team with your required volume for a custom quote.

Do you provide a Certificate of Analysis (CoA) for this product?

A Certificate of Analysis is available for most products upon request. Contact our support team with the lot number to obtain the relevant CoA.

FEN1 ELISA Kit (Human)

Product Specifications

Reproducibility

Mean Intra-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //

CAS Number

7732-18-5

Gene Name

Flap structure-specific endonuclease 1

Gene Aliases

DNase IV; FEN-1; flap endonuclease 1; Flap structure-specific endonuclease 1; Maturation factor 1; maturation factor-1; MF1; RAD2.

Gene ID

2237

Accession Number

NP_004102.1

Reactivity

Homo sapiens, Human

Target

Flap endonuclease 1

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Competitive

Assay Principle

Aviva Systems Biology FEN1 ELISA Kit (OKEH08213) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-FEN1 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated FEN1 and incubated. The FEN1 found in the sample or standards competes with the biotinylated FEN1 for limited binding sites on the immobilized anti-FEN1 antibody. Excess unbound biotinylated FEN1 and sample or standard FEN1 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated FEN1 captured in the well and inversely proportional to the amount of FEN1 which was contained in the sample or standard.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol