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AKR7A5 ELISA Kit (Mouse)

Product Specifications

Reproducibility

Mean Intra-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //

CAS Number

7732-18-5

Gene Name

Aldo-keto reductase family 7, member A5 (aflatoxin aldehyde reductase)

Gene Aliases

0610025K21Rik; A; Afar; Afar1; aflatoxin B1 aldehyde reductase member 2; Akr7a2; SSA reductase; succinic semialdehyde reductase.

Gene ID

110198

Accession Number

NP_079613.3

Reactivity

Mouse, Mus musculus

Target

Aflatoxin B1 aldehyde reductase member 2

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology AKR7A5 ELISA Kit (Mouse) (OKEH08199) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for AKR7A5 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for AKR7A5 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample AKR7A5 captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Detection Range

78-5000pg/mL

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 90% (n = 20)

Sensitivity

18pg/mL

Reconstitution

Store as indicated in product manual.

Specificity

Natural and recombinant Mouse Aflatoxin B1 aldehyde reductase member 2

NCBI Gene Symbol

Akr7a5

Protein Name

Q8CG76

Gene Name URL

Akr7a5

Nucleotide Accession Number

NM_025337.3

Frequently Asked Questions

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