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GK ELISA Kit (Human)

Product Specifications

Reproducibility

Mean Intra-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //

CAS Number

7732-18-5

Gene Name

Glycerol kinase

Gene Aliases

ATP:glycerol 3-phosphotransferase; GK1; GKD; glycerokinase; glycerol kinase.

Gene ID

2710

Accession Number

NP_000158.1

Reactivity

Homo sapiens, Human

Target

Glycerol kinase

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Competitive

Assay Principle

Aviva Systems Biology GK ELISA Kit (OKEH08183) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-GK antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated GK and incubated. The GK found in the sample or standards competes with the biotinylated GK for limited binding sites on the immobilized anti-GK antibody. Excess unbound biotinylated GK and sample or standard GK is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated GK captured in the well and inversely proportional to the amount of GK which was contained in the sample or standard.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Detection Range

1.56-100ng/mL

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 87% (n = 20)

Sensitivity

0.76ng/mL

Reconstitution

Store as indicated in product manual.

Specificity

Natural and recombinant Human Glycerol kinase

NCBI Gene Symbol

GK

Protein Name

P32189

Gene Name URL

GK

Nucleotide Accession Number

NM_000167.5

Frequently Asked Questions

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