BTLA ELISA Kit (Human)
Product Specifications
Reproducibility
Mean Intra-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //
CAS Number
7732-18-5
Gene Name
B and T lymphocyte associated
Gene Aliases
B- and T-lymphocyte attenuator; B- and T-lymphocyte-associated protein; BTLA1; CD272.
Gene ID
151888
Accession Number
NP_001078826.1
Reactivity
Homo sapiens, Human
Target
B- and T-lymphocyte attenuator
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Competitive
Assay Principle
Aviva Systems Biology BTLA ELISA Kit (OKEH08182) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-BTLA antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated BTLA and incubated. The BTLA found in the sample or standards competes with the biotinylated BTLA for limited binding sites on the immobilized anti-BTLA antibody. Excess unbound biotinylated BTLA and sample or standard BTLA is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated BTLA captured in the well and inversely proportional to the amount of BTLA which was contained in the sample or standard.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Detection Range
0.78-50ng/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 113% (n = 20)
Sensitivity
0.3ng/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Human B- and T-lymphocyte attenuator
NCBI Gene Symbol
BTLA
Protein Name
Q7Z6A9
Gene Name URL
BTLA
Nucleotide Accession Number
NM_001085357.1
Frequently Asked Questions
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