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DAXX ELISA Kit (Human)

Product Specifications

Reproducibility

Mean Intra-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //

CAS Number

7732-18-5

Gene Name

Death domain associated protein

Gene Aliases

BING2; CENP-C binding protein; DAP6; Daxx; death domain-associated protein 6; death-associated protein 6; EAP1; ETS1-associated protein 1; fas death domain-associated protein; Fas-binding protein; SMIM40.

Gene ID

1616

Accession Number

NP_001135441.1

Reactivity

Homo sapiens, Human

Target

Death domain-associated protein 6

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology DAXX ELISA Kit (Human) (OKEH08174) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for DAXX has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for DAXX is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample DAXX captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 98% (n = 20)

Reconstitution

Store as indicated in product manual.

Specificity

Natural and recombinant Human Death domain-associated protein 6

NCBI Gene Symbol

DAXX

Protein Name

Q9UER7

Gene Name URL

DAXX

Nucleotide Accession Number

NM_001141969.1

Frequently Asked Questions

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