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SERPINB10 ELISA Kit (Human)

Product Specifications

Reproducibility

Mean Intra-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //

CAS Number

7732-18-5

Gene Name

Serpin family B member 10

Gene Aliases

Bomapin; peptidase inhibitor 10; PI10; PI-10; protease inhibitor 10 (ovalbumin type) ; protease inhibitor 10 (ovalbumin type, bomapin) ; serine (or cysteine) proteinase inhibitor, clade B (ovalbumin), member 10; serpin B10; serpin peptidase inhibitor, clade B (ovalbumin), member 10.

Gene ID

5273

Accession Number

NP_005015.1

Reactivity

Homo sapiens, Human

Target

Serpin B10

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Competitive

Assay Principle

Aviva Systems Biology SERPINB10 ELISA Kit (OKEH08122) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-SERPINB10 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated SERPINB10 and incubated. The SERPINB10 found in the sample or standards competes with the biotinylated SERPINB10 for limited binding sites on the immobilized anti-SERPINB10 antibody. Excess unbound biotinylated SERPINB10 and sample or standard SERPINB10 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated SERPINB10 captured in the well and inversely proportional to the amount of SERPINB10 which was contained in the sample or standard.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Detection Range

0.78-50ng/mL

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 103% (n = 20)

Sensitivity

0.32ng/mL

Reconstitution

Store as indicated in product manual.

Specificity

Natural and recombinant Human Serpin B10

NCBI Gene Symbol

SERPINB10

Protein Name

P48595

Gene Name URL

SERPINB10

Nucleotide Accession Number

NM_005024.2

Frequently Asked Questions

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