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ATP2A1 ELISA Kit (Bovine)

Product Specifications

Reproducibility

Mean Intra-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //

CAS Number

7732-18-5

Gene Name

ATPase sarcoplasmic/endoplasmic reticulum Ca2+ transporting 1

Gene Aliases

ATPase, Ca++ transporting, fast twitch 1; calcium pump 1; Calcium-transporting ATPase sarcoplasmic reticulum type, fast twitch skeletal muscle isoform; endoplasmic reticulum class 1/2 Ca (2+) ATPase; sarcoplasmic/endoplasmic reticulum calcium ATPase 1; SERCA1; SR Ca (2+) -ATPase 1.

Gene ID

518117

Accession Number

NP_001069235.1

Reactivity

Bos taurus, Bovine

Target

Sarcoplasmic/endoplasmic reticulum calcium ATPase 1

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Competitive

Assay Principle

Aviva Systems Biology ATP2A1 ELISA Kit (OKEH08096) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-ATP2A1 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated ATP2A1 and incubated. The ATP2A1 found in the sample or standards competes with the biotinylated ATP2A1 for limited binding sites on the immobilized anti-ATP2A1 antibody. Excess unbound biotinylated ATP2A1 and sample or standard ATP2A1 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated ATP2A1 captured in the well and inversely proportional to the amount of ATP2A1 which was contained in the sample or standard.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Detection Range

0.78-50ng/mL

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 101% (n = 20)

Sensitivity

0.27ng/mL

Reconstitution

Store as indicated in product manual.

Specificity

Natural and recombinant Bovine Sarcoplasmic/endoplasmic reticulum calcium ATPase 1

NCBI Gene Symbol

ATP2A1

Protein Name

Q0VCY0

Gene Name URL

ATP2A1

Nucleotide Accession Number

NM_001075767.1

Frequently Asked Questions

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