ATP2A1 ELISA Kit (Bovine)
Product Specifications
Reproducibility
Mean Intra-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //
CAS Number
7732-18-5
Gene Name
ATPase sarcoplasmic/endoplasmic reticulum Ca2+ transporting 1
Gene Aliases
ATPase, Ca++ transporting, fast twitch 1; calcium pump 1; Calcium-transporting ATPase sarcoplasmic reticulum type, fast twitch skeletal muscle isoform; endoplasmic reticulum class 1/2 Ca (2+) ATPase; sarcoplasmic/endoplasmic reticulum calcium ATPase 1; SERCA1; SR Ca (2+) -ATPase 1.
Gene ID
518117
Accession Number
NP_001069235.1
Reactivity
Bos taurus, Bovine
Target
Sarcoplasmic/endoplasmic reticulum calcium ATPase 1
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Competitive
Assay Principle
Aviva Systems Biology ATP2A1 ELISA Kit (OKEH08096) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-ATP2A1 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated ATP2A1 and incubated. The ATP2A1 found in the sample or standards competes with the biotinylated ATP2A1 for limited binding sites on the immobilized anti-ATP2A1 antibody. Excess unbound biotinylated ATP2A1 and sample or standard ATP2A1 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated ATP2A1 captured in the well and inversely proportional to the amount of ATP2A1 which was contained in the sample or standard.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Detection Range
0.78-50ng/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 101% (n = 20)
Sensitivity
0.27ng/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Bovine Sarcoplasmic/endoplasmic reticulum calcium ATPase 1
NCBI Gene Symbol
ATP2A1
Protein Name
Q0VCY0
Gene Name URL
ATP2A1
Nucleotide Accession Number
NM_001075767.1
Frequently Asked Questions
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