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HIST1H46L2 ELISA Kit (Chicken)

Product Specifications

Reproducibility

Mean Intra-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //

CAS Number

7732-18-5

Gene Name

Histone cluster 1, H4-IV, germinal H4-like 2

Gene Aliases

DJ221C16.9; germinal histone H4; H1; H4; H4 (aa 1-103) ; H4 histone family, member A; H4 histone family, member B; H4/b; H4FB; H4/o; H4FA; H4-I; H4-II; H4-III; H4-IV; H4-V; H4-VI; H4-VII; H4-VIII; HIST1H46L1; HIST1H46L3; HIST1H46L4; HIST1H47; HIST1H4A; histone 1, H4; histone 1, H4a; histone 1, H4d; histone 2, H4b; histone cluster 1, H4a; histone cluster 1, H4-IV, germinal H4-like 1 (similar to human histone cluster 1, class H4 genes) ; histone cluster 1, H4-IV, germinal H4-like 2; histone cluster 1, H4-IV, germinal H4-like 2 (similar to human histone cluster 1, class H4 genes) ; histone cluster 1, H4-IV, germinal H4-like 3 (similar to human histone cluster 1, class H4 genes) ; histone cluster 1, H4-IV, germinal H4-like 4 (similar to human histone cluster 1, class H4 genes) ; histone cluster 1, H4-VI, germinal H4 (similar to human histone cluster 1, class H4 genes) ; histone cluster 1, histone class H4, member 7 (similar to human histone cluster 1, class H4 genes) ; histone H4; histone H4 (aa 1-103) ; histone H4 type VIII; histone H4-VI; histone H4-VII; histone H4-VIII; istone H4.

Gene ID

100858049, 100858319, 417946, 417950, 427884, 770005, 770142

Accession Number

NP_001032932.1

Reactivity

Chicken, Gallus gallus

Target

Histone H4

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology HIST1H46L2, HIST1H4I, HIST1H46, HIST2H4B, HIST1H4D, HIST1H4B, HIST1H46L6 ELISA Kit (Chicken) (OKEH08054) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for HIST1H46L2, HIST1H4I, HIST1H46, HIST2H4B, HIST1H4D, HIST1H4B, HIST1H46L6 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for HIST1H46L2, HIST1H4I, HIST1H46, HIST2H4B, HIST1H4D, HIST1H4B, HIST1H46L6 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample HIST1H46L2, HIST1H4I, HIST1H46, HIST2H4B, HIST1H4D, HIST1H4B, HIST1H46L6 captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Detection Range

0.156-10ng/mL

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 80% (n = 20)

Sensitivity

0.056ng/mL

Reconstitution

Store as indicated in product manual.

Specificity

Natural and recombinant Chicken Histone H4

NCBI Gene Symbol

HIST1H46, HIST1H46L2, HIST1H46L6, HIST1H4B, HIST1H4D, HIST1H4I, HIST2H4B

Protein Name

P62801

Gene Name URL

HIST1H46, HIST1H46L2, HIST1H46L6, HIST1H4B, HIST1H4D, HIST1H4I, HIST2H4B

Nucleotide Accession Number

NM_001037843.1

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