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SNTN ELISA Kit (Mouse)

Product Specifications

Reproducibility

Mean Intra-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //

CAS Number

7732-18-5

Gene Name

Sentan, cilia apical structure protein

Gene Aliases

A430083B19Rik; Protein S100-A1-like; S100 calcium-binding protein A1-like; sentan.

Gene ID

218739

Accession Number

NP_808292.1

Reactivity

Mouse, Mus musculus

Target

Sentan

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology SNTN ELISA Kit (Mouse) (OKEH08048) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for SNTN has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for SNTN is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample SNTN captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Detection Range

0.156-10 ng/mL

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 118% (n = 20)

Sensitivity

0.067 ng/mL

Reconstitution

Store as indicated in product manual.

Specificity

Natural and recombinant Mouse Sentan

NCBI Gene Symbol

Sntn

Protein Name

Q8C9X1

Gene Name URL

Sntn

Nucleotide Accession Number

NM_177624.3

Frequently Asked Questions

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