SNTN ELISA Kit (Human)
Product Specifications
Reproducibility
Mean Intra-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //
CAS Number
7732-18-5
Gene Name
Sentan, cilia apical structure protein
Gene Aliases
Protein S100-A1-like; S100 calcium-binding protein A1-like; S100A1L; S100AL; S100A-like protein; sentan.
Gene ID
132203
Accession Number
NP_001074006.1
Reactivity
Homo sapiens, Human
Target
Sentan
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Competitive
Assay Principle
Aviva Systems Biology SNTN ELISA Kit (OKEH08047) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-SNTN antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated SNTN and incubated. The SNTN found in the sample or standards competes with the biotinylated SNTN for limited binding sites on the immobilized anti-SNTN antibody. Excess unbound biotinylated SNTN and sample or standard SNTN is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated SNTN captured in the well and inversely proportional to the amount of SNTN which was contained in the sample or standard.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Detection Range
0.312-20ng/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 105% (n = 20)
Sensitivity
0.125ng/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Human Sentan
NCBI Gene Symbol
SNTN
Protein Name
A6NMZ2
Gene Name URL
SNTN
Nucleotide Accession Number
NM_001080537.1
Curated Selection
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