SNTN ELISA Kit (Human)

Product Specifications

Reproducibility

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CAS Number

7732-18-5

Gene Name

Sentan, cilia apical structure protein

Gene Aliases

Protein S100-A1-like; S100 calcium-binding protein A1-like; S100A1L; S100AL; S100A-like protein; sentan.

Gene ID

132203

Accession Number

NP_001074006.1

Reactivity

Homo sapiens, Human

Target

Sentan

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Competitive

Assay Principle

Aviva Systems Biology SNTN ELISA Kit (OKEH08047) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-SNTN antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated SNTN and incubated. The SNTN found in the sample or standards competes with the biotinylated SNTN for limited binding sites on the immobilized anti-SNTN antibody. Excess unbound biotinylated SNTN and sample or standard SNTN is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated SNTN captured in the well and inversely proportional to the amount of SNTN which was contained in the sample or standard.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol