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ACLY ELISA Kit (Bovine)

Product Specifications

Reproducibility

Mean Intra-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //

CAS Number

7732-18-5

Gene Name

ATP citrate lyase

Gene Aliases

ATP-citrate (pro-S-) -lyase; ATP-citrate synthase; citrate cleavage enzyme.

Gene ID

511135

Accession Number

NP_001032534.1

Reactivity

Bos taurus, Bovine

Target

ATP-citrate synthase

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology ACLY ELISA Kit (Bovine) (OKEH08002) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for ACLY has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for ACLY is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample ACLY captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 89% (n = 20)

Reconstitution

Store as indicated in product manual.

Specificity

Natural and recombinant Bovine ATP-citrate synthase

NCBI Gene Symbol

ACLY

Protein Name

Q32PF2

Gene Name URL

ACLY

Nucleotide Accession Number

NM_001037457.1

Frequently Asked Questions

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