Welcome to GenPrice! Check out our latest updates.

Shopping Cart (0)

Your cart is empty

Add some products to get started!

WNT1 ELISA Kit (Chicken)

Product Specifications

Reproducibility

Mean Intra-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //

CAS Number

7732-18-5

Gene Name

Wnt family member 1

Gene Aliases

Protein Wnt-1; wingless-type MMTV integration site family member 1; WNT-1.

Gene ID

396160

Accession Number

XP_015128668.1

Reactivity

Chicken, Gallus gallus

Target

Protein Wnt-1

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology WNT1 ELISA Kit (Chicken) (OKEH07992) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for WNT1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for WNT1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample WNT1 captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Detection Range

7.8-500pg/mL

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 85% (n = 20)

Sensitivity

3.3pg/mL

Reconstitution

Store as indicated in product manual.

Specificity

Natural and recombinant Chicken Protein Wnt-1

NCBI Gene Symbol

WNT1

Protein Name

Q91029

Gene Name URL

WNT1

Nucleotide Accession Number

XM_015273182.1

Frequently Asked Questions

More Discoveries

Explore Other Products

Browse additional items from our catalog

Human MSH5 Knockout HEK293T cell line
GTR15420635 1 Vial

Human MSH5 Knockout HEK293T cell line

Sign In for Pricing
View Details
Angiopoietin 1 (Agpt, ang1, Ang1, Ang-1, ANG-1, Angiopoietin-1) (APC)
A2293-01D-APC 100 µL

Angiopoietin 1 (Agpt, ang1, Ang1, Ang-1, ANG-1, Angiopoietin-1) (APC)

Sign In for Pricing
View Details
Recombinant Human TGF-beta 1
RP0006-01 50 µg

Recombinant Human TGF-beta 1

Sign In for Pricing
View Details
Recombinant Human TGF-beta 1
RP0006-02 500 µg

Recombinant Human TGF-beta 1

Sign In for Pricing
View Details
Recombinant Human TGF-beta 1
RP0006-03 1000 µg

Recombinant Human TGF-beta 1

Sign In for Pricing
View Details
Defensin α3 Polyclonal Antibody
E20-71323 100 µg

Defensin α3 Polyclonal Antibody

Sign In for Pricing
View Details