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ACKR2 ELISA Kit (Human)

Product Specifications

Reproducibility

Mean Intra-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //

Gene Name

Atypical chemokine receptor 2

Gene Aliases

Atypical chemokine receptor 2; C-C chemokine receptor D6; CCBP2; CC-chemokine-binding receptor JAB61; CCR10; CCR9; chemokine (C-C motif) receptor 9; chemokine (C-C) receptor 9; chemokine receptor CCR-10; chemokine receptor CCR-9; chemokine receptor D6; chemokine-binding protein 2; chemokine-binding protein D6; CMKBR9; D6; hD6.

Gene ID

1238

Accession Number

NP_001287.2

Reactivity

Homo sapiens, Human

Target

Atypical chemokine receptor 2

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology ACKR2 ELISA Kit (Human) (OKEH07984) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for ACKR2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for ACKR2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample ACKR2 captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Detection Range

31.2-2000pg/mL

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 113% (n = 20)

Sensitivity

9.5pg/mL

Reconstitution

Store as indicated in product manual.

Specificity

Natural and recombinant Human Atypical chemokine receptor 2

NCBI Gene Symbol

ACKR2

Protein Name

O00590

Gene Name URL

ACKR2

CAS Number

7732-18-5

Nucleotide Accession Number

NM_001296.4

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