ITIH4 ELISA Kit (Bovine)

Product Specifications

Reproducibility

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CAS Number

7732-18-5

Gene Name

Inter-alpha-trypsin inhibitor heavy chain 4

Gene Aliases

Inter-alpha (globulin) inhibitor H4 (plasma Kallikrein-sensitive glycoprotein) ; inter-alpha-inhibitor heavy chain 4; inter-alpha-trypsin inhibitor heavy chain family member 4; inter-alpha-trypsin inhibitor heavy chain H4; ITI heavy chain H4; ITI-HC4.

Gene ID

513700

Accession Number

NP_001015590.2

Reactivity

Bos taurus, Bovine

Target

Inter-alpha-trypsin inhibitor heavy chain H4

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Competitive

Assay Principle

Aviva Systems Biology ITIH4 ELISA Kit (OKEH07949) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-ITIH4 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated ITIH4 and incubated. The ITIH4 found in the sample or standards competes with the biotinylated ITIH4 for limited binding sites on the immobilized anti-ITIH4 antibody. Excess unbound biotinylated ITIH4 and sample or standard ITIH4 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated ITIH4 captured in the well and inversely proportional to the amount of ITIH4 which was contained in the sample or standard.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol