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METTL3 ELISA Kit (Human)

Product Specifications

Reproducibility

Mean Intra-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //

CAS Number

7732-18-5

Gene Name

Methyltransferase 3, N6-adenosine-methyltransferase complex catalytic subunit

Gene Aliases

AdoMet-binding subunit of the human mRNA (N6-adenosine) -methyltransferase; hMETTL3; IME4; M6A; methyltransferase like 3; methyltransferase-like protein 3; mRNA (2'-O-methyladenosine-N (6) -) -methyltransferase; mRNA m (6) A methyltransferase; MT-A70; N6-adenosine-methyltransferase 70 kDa subunit; N6-adenosine-methyltransferase catalytic subunit; Spo8.

Gene ID

56339

Accession Number

NP_062826.2

Reactivity

Homo sapiens, Human

Target

N6-adenosine-methyltransferase catalytic subunit

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology METTL3 ELISA Kit (Human) (OKEH07942) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for METTL3 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for METTL3 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample METTL3 captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Detection Range

0.312-20ng/mL

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 101% (n = 20)

Sensitivity

0.178ng/mL

Reconstitution

Store as indicated in product manual.

Specificity

Natural and recombinant Human N6-adenosine-methyltransferase catalytic subunit

NCBI Gene Symbol

METTL3

Protein Name

Q86U44

Gene Name URL

METTL3

Nucleotide Accession Number

NM_019852.4

Frequently Asked Questions

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