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PITX2 ELISA Kit (Mouse)

Product Specifications

Reproducibility

Mean Intra-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //

CAS Number

7732-18-5

Gene Name

Paired-like homeodomain transcription factor 2

Gene Aliases

9430085M16Rik; ALL1-responsive protein ARP1; Brx; Brx1; BRX1 homeoprotein; Brx1b; Homeobox protein PITX2; Munc3; Munc30; orthodenticle-like homeobox 2; Otl; Otlx2; Paired-like homeodomain transcription factor 2; paired-like homeodomain transcription factor Munc 30; Pit; pituitary homeobox 2; Pitx; Ptx; Ptx2; Ri; Rieg; so; solurshin.

Gene ID

18741

Accession Number

NP_001035967.1

Reactivity

Mouse, Mus musculus

Target

Pituitary homeobox 2

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology PITX2 ELISA Kit (Mouse) (OKEH07925) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for PITX2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for PITX2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample PITX2 captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Detection Range

0.156-10 ng/ml

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 104% (n = 20)

Sensitivity

0.078 ng/mL

Reconstitution

Store as indicated in product manual.

Specificity

Natural and recombinant Mouse Pituitary homeobox 2

NCBI Gene Symbol

Pitx2

Protein Name

P97474

Gene Name URL

Pitx2

Nucleotide Accession Number

NM_001042502.2

Frequently Asked Questions

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