CEMIP2 ELISA Kit (Human)
Product Specifications
Reproducibility
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CAS Number
7732-18-5
Gene Name
Cell migration inducing hyaluronidase 2
Gene Aliases
Cell surface hyaluronidase; TMEM2; transmembrane protein 2.
Gene ID
23670
Accession Number
NP_001129292.1
Reactivity
Homo sapiens, Human
Target
Cell surface hyaluronidase
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology CEMIP2 ELISA Kit (Human) (OKEH07897) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CEMIP2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CEMIP2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CEMIP2 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Detection Range
78-5000pg/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 101% (n = 20)
Sensitivity
39.8pg/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Human Cell surface hyaluronidase
NCBI Gene Symbol
CEMIP2
Protein Name
Q9UHN6
Gene Name URL
CEMIP2
Nucleotide Accession Number
NM_001135820.1
Frequently Asked Questions
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