TRRAP ELISA Kit (Human)
Product Specifications
Reproducibility
Mean Intra-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //
Gene Name
Transformation/transcription domain associated protein
Gene Aliases
350/400 kDa PCAF-associated factor; DEDDFA; DFNA75; PAF350/400; PAF400; STAF40; Tra1; tra1 homolog; transformation/transcription domain-associated protein; TR-AP.
Gene ID
8295
Accession Number
NP_001231509.1
Reactivity
Homo sapiens, Human
Target
Transformation/transcription domain-associated protein
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology TRRAP ELISA Kit (Human) (OKEH07896) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for TRRAP has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for TRRAP is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample TRRAP captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Detection Range
0.156-10ng/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 111% (n = 20)
Sensitivity
0.062ng/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Human Transformation/transcription domain-associated protein
NCBI Gene Symbol
TRRAP
Protein Name
Q9Y4A5
Gene Name URL
TRRAP
CAS Number
7732-18-5
Nucleotide Accession Number
NM_001244580.1
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