UGT1A4 ELISA Kit (Human)
Product Specifications
Reproducibility
Mean Intra-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //
CAS Number
7732-18-5
Gene Name
UDP glucuronosyltransferase family 1 member A4
Gene Aliases
Bilirubin UDP-glucuronosyltransferase isozyme 2; Bilirubin-specific UDPGT isozyme 1; bilirubin-specific UDPGT isozyme 2; GNT1; hUG-BR1; HUG-BR2; UDP glucuronosyltransferase 1 family, polypeptide A4; UDP glycosyltransferase 1 family, polypeptide A4; UDP-glucuronosyltransferase 1 family polypeptide A4s; UDP-glucuronosyltransferase 1-1; UDP-glucuronosyltransferase 1-4; UDP-glucuronosyltransferase 1-A; UDP-glucuronosyltransferase 1A1; UDP-glucuronosyltransferase 1A4; UDP-glucuronosyltransferase 1-D; UDPGT; UDPGT 1-1; UDPGT 1-4; UGT1; UGT1.1; UGT1.4; UGT1-01; UGT1-04; UGT1A; UGT-1A; UGT1A1; UGT1A4S; UGT1D; UGT-1D.
Gene ID
54657
Accession Number
NP_009051.1
Reactivity
Homo sapiens, Human
Target
UDP-glucuronosyltransferase 1-4
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology UGT1A4 ELISA Kit (Human) (OKEH07890) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for UGT1A4 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for UGT1A4 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample UGT1A4 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Detection Range
78-5000pg/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 92% (n = 20)
Sensitivity
26pg/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Human UDP-glucuronosyltransferase 1-4
NCBI Gene Symbol
UGT1A4
Protein Name
P22310
Gene Name URL
UGT1A4
Nucleotide Accession Number
NM_007120.2
Frequently Asked Questions
More Discoveries
Explore Other Products
Browse additional items from our catalog