TINAGL1 ELISA Kit (Human)
Product Specifications
Reproducibility
Mean Intra-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //
CAS Number
7732-18-5
Gene Name
Tubulointerstitial nephritis antigen like 1
Gene Aliases
Androgen-regulated gene 1; ARG1; glucocorticoid-inducible protein 5; LCN7; LIECG3; lipocalin 7; OLRG-2; Oxidized LDL-responsive gene 2 protein; oxidized-LDL responsive gene 2; P3ECSL; TIN Ag-related protein; TINAG-like 1; TIN-Ag-RP; TINAGRP; tubulointerstitial nephritis antigen-like; tubulointerstitial nephritis antigen-related protein.
Gene ID
64129
Accession Number
NP_001191343.1
Reactivity
Homo sapiens, Human
Target
Tubulointerstitial nephritis antigen-like
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology TINAGL1 ELISA Kit (OKEH07868) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-TINAGL1 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated TINAGL1 and incubated. The TINAGL1 found in the sample or standards competes with the biotinylated TINAGL1 for limited binding sites on the immobilized anti-TINAGL1 antibody. Excess unbound biotinylated TINAGL1 and sample or standard TINAGL1 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated TINAGL1 captured in the well and inversely proportional to the amount of TINAGL1 which was contained in the sample or standard.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Detection Range
0.78-50ng/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 103% (n = 20)
Sensitivity
0.34 ng/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Human Tubulointerstitial nephritis antigen-like
NCBI Gene Symbol
TINAGL1
Protein Name
Q9GZM7
Gene Name URL
TINAGL1
Nucleotide Accession Number
NM_001204414.1
Frequently Asked Questions
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