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RTN4 ELISA Kit (Rat)

Product Specifications

Reproducibility

Mean Intra-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //

CAS Number

7732-18-5

Gene Name

Reticulon 4

Gene Aliases

Foocen; glut4 vesicle 20 kDa protein; GLUT4 vesicle 20kDa protein; neurite outgrowth inhibitor; NI-220/250; NI-250; Nogo; Nogo-A; r; rat N; rat NogoA; reticulon-4; Vp20.

Gene ID

83765

Accession Number

NP_114019.1

Reactivity

Rat, Rattus norvegicus

Target

Reticulon-4

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology RTN4 ELISA Kit (Rat) (OKEH07826) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for RTN4 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for RTN4 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample RTN4 captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Detection Range

78-5000pg/mL

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 86% (n = 20)

Sensitivity

40.6pg/mL

Reconstitution

Store as indicated in product manual.

Specificity

Natural and recombinant Rat Reticulon-4

NCBI Gene Symbol

Rtn4

Protein Name

Q9JK11

Gene Name URL

Rtn4

Nucleotide Accession Number

NM_031831.1

Frequently Asked Questions

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