What is the catalog number for DIO1 ELISA Kit (Chicken)?

The catalog number for DIO1 ELISA Kit (Chicken) is OKEH07820-96W.

What sizes are available for DIO1 ELISA Kit (Chicken)?

DIO1 ELISA Kit (Chicken) is available in the following size(s): 96 Tests.

Is DIO1 ELISA Kit (Chicken) in stock?

DIO1 ELISA Kit (Chicken) is currently made to order / not held in immediate stock. Contact us for lead time.

Is this product animal-origin-free?

Animal-origin-free status, where applicable, is noted on the datasheet under formulation details.

Does this product need any special customs documentation to be imported to my country?

If you’re outside the EU, UK, or USA, you’re responsible for customs clearance and any related taxes or fees. We provide the standard import documents, such as the Commercial Invoice, Packing List, and Airway Bill, in advance, so let us know early if your customs office needs anything additional for this specific product.

What buffer is this product supplied in?

The formulation or storage buffer is listed on the product datasheet. If you require a carrier-free or custom formulation, contact us before ordering, as this may involve a made-to-order production timeline.

DIO1 ELISA Kit (Chicken)

Product Specifications

Reproducibility

Mean Intra-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //

CAS Number

7732-18-5

Gene Name

Deiodinase, iodothyronine type I

Gene Aliases

5DI; DIOI; iodothyronine deiodinase 1; type 1 DI; type I iodothyronine deiodinase; type-I 5'-deiodinase.

Gene ID

395940

Accession Number

NP_001091083.1

Reactivity

Chicken, Gallus gallus

Target

Type I iodothyronine deiodinase

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology DIO1 ELISA Kit (Chicken) (OKEH07820) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for DIO1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for DIO1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample DIO1 captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Detection Range

0.156-10ng/mL

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 92% (n = 20)

Sensitivity

0.056ng/mL

Reconstitution

Store as indicated in product manual.

Specificity

Natural and recombinant Chicken Type I iodothyronine deiodinase

NCBI Gene Symbol

DIO1

Protein Name

O42411

Gene Name URL

DIO1

Nucleotide Accession Number

NM_001097614.1

Frequently Asked Questions

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