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TMPRSS3 ELISA Kit (Human)

Selected Variant

OKEH07809-96W

Size: 96 Tests

Product Specifications

Reproducibility

Mean Intra-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //

CAS Number

7732-18-5

Gene Name

Transmembrane serine protease 3

Gene Aliases

DFNB10; DFNB8; ECHOS1; serine protease TADG-12; TADG12; transmembrane protease serine 3; transmembrane protease, serine 3; tumor-associated differentially-expressed gene 12 protein.

Gene ID

64699

Accession Number

NP_001243246.1

Reactivity

Homo sapiens, Human

Target

Transmembrane protease serine 3

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology TMPRSS3 ELISA Kit (Human) (OKEH07809) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for TMPRSS3 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for TMPRSS3 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample TMPRSS3 captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Detection Range

0.156-10ng/mL

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 117% (n = 20)

Sensitivity

0.056ng/mL

Reconstitution

Store as indicated in product manual.

Specificity

Natural and recombinant Human Transmembrane protease serine 3

NCBI Gene Symbol

TMPRSS3

Protein Name

P57727

Gene Name URL

TMPRSS3

Nucleotide Accession Number

NM_001256317.1

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