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FBXO32 ELISA Kit (Mouse)

Selected Variant

OKEH07775-96W

Size: 96 Tests

Product Specifications

Reproducibility

Mean Intra-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //

CAS Number

7732-18-5

Gene Name

F-box protein 32

Gene Aliases

4833442G10Rik; AI430017; ATR; atro; atrogin 1; Atrogin-1; ATROGIN1; F-box only protein 32; Gm20361; MAFb; MAFbx; muscle atrophy F-box protein.

Gene ID

67731

Accession Number

NP_080622.1

Reactivity

Mouse, Mus musculus

Target

F-box only protein 32

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology FBXO32 ELISA Kit (Mouse) (OKEH07775) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for FBXO32 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for FBXO32 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample FBXO32 captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Detection Range

15.6-1000pg/mL

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 90% (n = 20)

Sensitivity

7.86pg/mL

Reconstitution

Store as indicated in product manual.

Specificity

Natural and recombinant Mouse F-box only protein 32

NCBI Gene Symbol

Fbxo32

Protein Name

Q9CPU7

Gene Name URL

Fbxo32

Nucleotide Accession Number

NM_026346.3

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