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CALCRL ELISA Kit (Human)

Product Specifications

Reproducibility

Mean Intra-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //

CAS Number

7732-18-5

Gene Name

Calcitonin receptor like receptor

Gene Aliases

Calcitonin gene-related peptide type 1 receptor; calcitonin receptor-like; Calcitonin receptor-like receptor; CGRP type 1 receptor; CGRPR; CRLR; LMPHM8.

Gene ID

10203

Accession Number

NP_001258680.1

Reactivity

Homo sapiens, Human

Target

Calcitonin gene-related peptide type 1 receptor

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Competitive

Assay Principle

Aviva Systems Biology CALCRL ELISA Kit (OKEH07763) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-CALCRL antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated CALCRL and incubated. The CALCRL found in the sample or standards competes with the biotinylated CALCRL for limited binding sites on the immobilized anti-CALCRL antibody. Excess unbound biotinylated CALCRL and sample or standard CALCRL is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated CALCRL captured in the well and inversely proportional to the amount of CALCRL which was contained in the sample or standard.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Detection Range

0.78-50ng/mL

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 82% (n = 20)

Sensitivity

0.36ng/mL

Reconstitution

Store as indicated in product manual.

Specificity

Natural and recombinant Human Calcitonin gene-related peptide type 1 receptor

NCBI Gene Symbol

CALCRL

Protein Name

Q16602

Gene Name URL

CALCRL

Nucleotide Accession Number

NM_001271751.1

Frequently Asked Questions

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