Welcome to GenPrice! Check out our latest updates.

Shopping Cart (0)

Your cart is empty

Add some products to get started!

CLDN5 ELISA Kit (Human)

Product Specifications

Reproducibility

Mean Intra-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //

CAS Number

7732-18-5

Gene Name

Claudin 5

Gene Aliases

AWAL; BEC1; claudin-5; CPETRL1; TMDVCF; TMVCF; transmembrane protein deleted in VCFS; transmembrane protein deleted in velocardiofacial syndrome.

Gene ID

7122

Accession Number

NP_001124333.1

Reactivity

Homo sapiens, Human

Target

Claudin-5

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology CLDN5 ELISA Kit (Human) (OKEH07757) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CLDN5 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CLDN5 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CLDN5 captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Detection Range

1.56-100ng/mL

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 117% (n = 20)

Sensitivity

0.78ng/mL

Reconstitution

Store as indicated in product manual.

Specificity

Natural and recombinant Human Claudin-5

NCBI Gene Symbol

CLDN5

Protein Name

O00501

Gene Name URL

CLDN5

Nucleotide Accession Number

NM_001130861.1

Frequently Asked Questions

More Discoveries

Explore Other Products

Browse additional items from our catalog

Rat Hk2/Hexokinase-2 ELISA Kit
MBS2890746-01 48 Well

Rat Hk2/Hexokinase-2 ELISA Kit

Sign In for Pricing
View Details
Rat Hk2/Hexokinase-2 ELISA Kit
MBS2890746-02 96 Well

Rat Hk2/Hexokinase-2 ELISA Kit

Sign In for Pricing
View Details
Rat Hk2/Hexokinase-2 ELISA Kit
MBS2890746-03 5x 96 Well

Rat Hk2/Hexokinase-2 ELISA Kit

Sign In for Pricing
View Details
Rat Hk2/Hexokinase-2 ELISA Kit
MBS2890746-04 10x 96 Well

Rat Hk2/Hexokinase-2 ELISA Kit

Sign In for Pricing
View Details
Human CORIN qPCR Primer Pair
QH32201S 200 Tests

Human CORIN qPCR Primer Pair

Sign In for Pricing
View Details
Recombinant Dinoroseobacter shibae Glutamate--tRNA ligase 1 (gltX1)
MBS1037742-01 0.02 mg (E-Coli)

Recombinant Dinoroseobacter shibae Glutamate--tRNA ligase 1 (gltX1)

Sign In for Pricing
View Details