What is the catalog number for CLDN5 ELISA Kit (Human)?

The catalog number for CLDN5 ELISA Kit (Human) is OKEH07757-96W.

What sizes are available for CLDN5 ELISA Kit (Human)?

CLDN5 ELISA Kit (Human) is available in the following size(s): 96 Tests.

Is CLDN5 ELISA Kit (Human) in stock?

CLDN5 ELISA Kit (Human) is currently made to order / not held in immediate stock. Contact us for lead time.

Can this product be used for diagnostic or therapeutic purposes?

No. This product, like all items we supply, is intended for research laboratory use only and must not be used for diagnostics, therapeutics, treatment, or consumption by humans or animals.

What is the shelf life of this product?

Shelf life and expiration dates vary by product and are shown on the product listing and label. Products used beyond their expiration date are not eligible for performance-related returns or refunds, so please check the lot number and expiry before use.

What expression system was used for this recombinant protein?

The expression system, such as E. coli, HEK293, or insect cells, is listed on the datasheet under production details, which can affect post-translational modifications relevant to your application.

CLDN5 ELISA Kit (Human)

Selected Variant

OKEH07757-96W

Size: 96 Tests

Product Specifications

Reproducibility

Mean Intra-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //

CAS Number

7732-18-5

Gene Name

Claudin 5

Gene Aliases

AWAL; BEC1; claudin-5; CPETRL1; TMDVCF; TMVCF; transmembrane protein deleted in VCFS; transmembrane protein deleted in velocardiofacial syndrome.

Gene ID

7122

Accession Number

NP_001124333.1

Reactivity

Homo sapiens, Human

Target

Claudin-5

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology CLDN5 ELISA Kit (Human) (OKEH07757) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CLDN5 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CLDN5 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CLDN5 captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Detection Range

1.56-100ng/mL

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 117% (n = 20)

Sensitivity

0.78ng/mL

Reconstitution

Store as indicated in product manual.

Specificity

Natural and recombinant Human Claudin-5

NCBI Gene Symbol

CLDN5

Protein Name

O00501

Gene Name URL

CLDN5

Nucleotide Accession Number

NM_001130861.1

Price

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