CEP290 ELISA Kit (Human)
Product Specifications
Reproducibility
Mean Intra-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //
CAS Number
7732-18-5
Gene Name
Centrosomal protein 290
Gene Aliases
3H11Ag; Bardet-Biedl syndrome 14 protein; BBS14; cancer/testis antigen 87; centrosomal protein 290kDa; centrosomal protein of 290 kDa; CT87; CTCL tumor antigen se2-2; JBTS5; LCA10; Meckel syndrome, type 4; MKS4; monoclonal antibody 3H11 antigen; Nephrocystin-6; nephrocytsin-6; NPHP6; POC3; POC3 centriolar protein homolog; prostate cancer antigen T21; rd16; SLSN6; tumor antigen se2-2.
Gene ID
80184
Accession Number
NP_079390.3
Reactivity
Homo sapiens, Human
Target
Centrosomal protein of 290 kDa
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology CEP290 ELISA Kit (Human) (OKEH07751) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CEP290 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CEP290 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CEP290 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Detection Range
0.78-50ng/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 109% (n = 20)
Sensitivity
0.25ng/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Human Centrosomal protein of 290 kDa
NCBI Gene Symbol
CEP290
Protein Name
O15078
Gene Name URL
CEP290
Nucleotide Accession Number
NM_025114.3
Frequently Asked Questions
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