ENPP3 ELISA Kit (Human)
Product Specifications
Reproducibility
Mean Intra-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //
CAS Number
7732-18-5
Gene Name
Ectonucleotide pyrophosphatase/phosphodiesterase 3
Gene Aliases
B10; CD203c; dJ1005H11.3 (phosphodiesterase I/nucleotide pyrophosphatase 3) ; dJ914N13.3 (phosphodiesterase I/nucleotide pyrophosphatase 3) ; ectonucleotide pyrophosphatase/phosphodiesterase family member 3; gp130RB13-6; NPP3; PD-IBETA; PDNP3; Phosphodiesterase I beta; phosphodiesterase I/nucleotide pyrophosphatase 3; phosphodiesterase-I beta.
Gene ID
5169
Accession Number
NP_005012.2
Reactivity
Homo sapiens, Human
Target
Ectonucleotide pyrophosphatase/phosphodiesterase family member 3
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology ENPP3 ELISA Kit (Human) (OKEH07749) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for ENPP3 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for ENPP3 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample ENPP3 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Detection Range
0.78-50ng/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 82% (n = 20)
Sensitivity
0.38ng/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Human Ectonucleotide pyrophosphatase/phosphodiesterase family member 3
NCBI Gene Symbol
ENPP3
Protein Name
O14638
Gene Name URL
ENPP3
Nucleotide Accession Number
NM_005021.4
Frequently Asked Questions
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