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HSD11B2 ELISA Kit (Human)

Product Specifications

Reproducibility

Mean Intra-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //

CAS Number

7732-18-5

Gene Name

Hydroxysteroid 11-beta dehydrogenase 2

Gene Aliases

11-beta-HSD type II;11-beta-HSD2;11-beta-hydroxysteroid dehydrogenase type 2;11-beta-hydroxysteroid dehydrogenase type II;11-DH2;11-HSD type II; AME; AME1; corticosteroid 11-beta-dehydrogenase isozyme 2; -HSD11 type II; HSD11K; HSD2; NAD-dependent 11-beta-hydroxysteroid dehydrogenase; SDR9C3; short chain dehydrogenase/reductase family 9C member 3.

Gene ID

3291

Accession Number

NP_000187.3

Reactivity

Homo sapiens, Human

Target

Corticosteroid 11-beta-dehydrogenase isozyme 2

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology HSD11B2 ELISA Kit (Human) (OKEH07746) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for HSD11B2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for HSD11B2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample HSD11B2 captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Detection Range

78-5000pg/mL

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 118% (n = 20)

Sensitivity

39pg/mL

Reconstitution

Store as indicated in product manual.

Specificity

Natural and recombinant Human Corticosteroid 11-beta-dehydrogenase isozyme 2

NCBI Gene Symbol

HSD11B2

Protein Name

P80365

Gene Name URL

HSD11B2

Nucleotide Accession Number

NM_000196.3

Frequently Asked Questions

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