SFPQ ELISA Kit (Human)
Product Specifications
Reproducibility
Mean Intra-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //
CAS Number
7732-18-5
Gene Name
Splicing factor proline and glutamine rich
Gene Aliases
100 kDa DNA-pairing protein; DNA-binding p52/p100 complex, 100 kDa subunit; epididymis secretory sperm binding protein; polypyrimidine tract binding protein associated; polypyrimidine tract-binding protein-associated splicing factor; Polypyrimidine tract-binding protein-associated-splicing factor; POMP100; PPP1R140; protein phosphatase 1, regulatory subunit 140; PSF; PTB-associated splicing factor; splicing factor proline/glutamine rich (polypyrimidine tract binding protein associated) ; splicing factor proline/glutamine-rich; splicing factor, proline- and glutamine-rich.
Gene ID
6421
Accession Number
NP_005057.1
Reactivity
Homo sapiens, Human
Target
Splicing factor, proline- and glutamine-rich
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology SFPQ ELISA Kit (Human) (OKEH07743) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for SFPQ has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for SFPQ is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample SFPQ captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Detection Range
0.156-10ng/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 92% (n = 20)
Sensitivity
0.08ng/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Human Splicing factor, proline- and glutamine-rich
NCBI Gene Symbol
SFPQ
Protein Name
P23246
Gene Name URL
SFPQ
Nucleotide Accession Number
NM_005066.2
Frequently Asked Questions
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