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SFPQ ELISA Kit (Human)

Product Specifications

Reproducibility

Mean Intra-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //

CAS Number

7732-18-5

Gene Name

Splicing factor proline and glutamine rich

Gene Aliases

100 kDa DNA-pairing protein; DNA-binding p52/p100 complex, 100 kDa subunit; epididymis secretory sperm binding protein; polypyrimidine tract binding protein associated; polypyrimidine tract-binding protein-associated splicing factor; Polypyrimidine tract-binding protein-associated-splicing factor; POMP100; PPP1R140; protein phosphatase 1, regulatory subunit 140; PSF; PTB-associated splicing factor; splicing factor proline/glutamine rich (polypyrimidine tract binding protein associated) ; splicing factor proline/glutamine-rich; splicing factor, proline- and glutamine-rich.

Gene ID

6421

Accession Number

NP_005057.1

Reactivity

Homo sapiens, Human

Target

Splicing factor, proline- and glutamine-rich

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology SFPQ ELISA Kit (Human) (OKEH07743) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for SFPQ has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for SFPQ is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample SFPQ captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Detection Range

0.156-10ng/mL

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 92% (n = 20)

Sensitivity

0.08ng/mL

Reconstitution

Store as indicated in product manual.

Specificity

Natural and recombinant Human Splicing factor, proline- and glutamine-rich

NCBI Gene Symbol

SFPQ

Protein Name

P23246

Gene Name URL

SFPQ

Nucleotide Accession Number

NM_005066.2

Frequently Asked Questions

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