GDF10 ELISA Kit (Bovine)

Product Specifications

Reproducibility

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CAS Number

7732-18-5

Gene Name

Growth differentiation factor 10

Gene Aliases

BMP-3b; bone morphogenetic protein 3b; GDF-10; growth/differentiation factor 10.

Gene ID

539510

Accession Number

NP_001069635.1

Reactivity

Bos taurus, Bovine

Target

Growth/differentiation factor 10

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Competitive

Assay Principle

Aviva Systems Biology GDF10 ELISA Kit (OKEH07738) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-GDF10 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated GDF10 and incubated. The GDF10 found in the sample or standards competes with the biotinylated GDF10 for limited binding sites on the immobilized anti-GDF10 antibody. Excess unbound biotinylated GDF10 and sample or standard GDF10 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated GDF10 captured in the well and inversely proportional to the amount of GDF10 which was contained in the sample or standard.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol